Training in Biotechnology
Sample Schedule

Day 1

Lecture: Introduction to biotechnology; the scientific method; fundamentals of measurement techniques and graphing; DNA extraction protocol
Lab: Equipment introductions: practice using micropipettes, making solutions, and casting agarose gels; DNA extraction

Day 2

Lecture: Introduction to molecular genetics; the central dogma of molecular biology; the genetics of viruses; restriction enzymes; gel electrophoresis protocol
Lab: Restriction digestion and electrophoresis of lambda DNA

Day 3

Lecture: Restriction-fragment length polymorphisms (RFLPs) and forensic analysis of DNA samples; polymerase chain reaction (PCR) protocol
Lab: DNA fingerprinting for forensic analysis

Day 4

Lecture: Introduction to genetics of bacteria; bacterial transformation; transformation protocol
Lab: Transformation of E. coli with pGLO jellyfish gene

Day 5

Lecture: Protein structure and function; chromatography protocol
Lab: Size exclusion chromatography (SEC) of protein mixture

Day 6

Lecture: Structure and function of green fluorescent protein (GFP); hydrophobic interaction chromatography (HIC) protocol
Lab: Isolation of GFP using HIC

Day 7

Lecture: Antibody structure and function; emerging viruses; enzyme-linked immunosorbent assay (ELISA) protocol
Lab: ELISA antibody detection of H1N1

Day 8

Lecture: Cloning and genetically modified organisms (GMOs)
Lab: Detection of GMO foods

Day 9

Lecture: Evolution of fish; cladistic analysis; protein fingerprinting protocol
Lab: Protein fingerprinting of fish proteins

Day 10

Lecture: The future of bioengineering; stem cell research
Lab: Biotech olympics